Journal: ACS Biomaterials Science & Engineering

Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers

doi: 10.1021/acsbiomaterials.5c02175

Figure Lengend Snippet: Basal expression of MCT and MPC in PC3 and FaDu cells. Representative immunoblots (a) and densitometric quantification (d) of basal MCT1, MCT4, and MPC expressions in PC3 and FaDu cells. Representative immunoblots (b) and quantification (e) show MCT1, MCT4, and MPC expression in PC3 cells under normoxia (21% O 2 ) or hypoxia (0.5% O 2 ), while representative immunoblots (c) and quantification (f) show the same proteins in FaDu cells under these conditions. Data represent the mean ± SD from three independent experiments. Statistical significance was determined using a two-tailed Student’s t test.

Article Snippet: FaDu (HPV(−) HNSCC) and PC3 (prostate cancer) cell lines were sourced from the American Type Culture Collection (ATCC) and routinely tested for mycoplasma contamination (Lonza).

Techniques: Expressing, Western Blot, Two Tailed Test

Journal: ACS Biomaterials Science & Engineering

Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers

doi: 10.1021/acsbiomaterials.5c02175

Figure Lengend Snippet: Inhibitors of lactate metabolism influence tumor cell growth depending on substrate availability (lactate or glucose). PC3 and FaDu cells were treated with AZD3965 (1 μM), syrosingopine (10 μM), 7ACC2 (10 μM), or in combination in complete media supplemented with either lactate or glucose for 72 h under normoxic (a–b) and hypoxic (c–d) conditions. Data represent mean ± SD from three independent replicates. Statistical significance was assessed using one-way ANOVA with Tukey’s posthoc test.

Article Snippet: FaDu (HPV(−) HNSCC) and PC3 (prostate cancer) cell lines were sourced from the American Type Culture Collection (ATCC) and routinely tested for mycoplasma contamination (Lonza).

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers

doi: 10.1021/acsbiomaterials.5c02175

Figure Lengend Snippet: Inhibiting lactate metabolism sensitizes tumor cells to radiation under both normoxic and hypoxic conditions. Clonogenic survival curves for PC3 (a, b) and FaDu (c, d) cells treated with AZD3965 (1 μM), syrosingopine (10 μM), 7ACC2 (10 μM), or combinations for 24 h under normoxia (a, c21% O 2 ) or hypoxia (b, d0.5% O 2 ). Data represent the mean ± SD of three independent experiments. Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparison test.

Article Snippet: FaDu (HPV(−) HNSCC) and PC3 (prostate cancer) cell lines were sourced from the American Type Culture Collection (ATCC) and routinely tested for mycoplasma contamination (Lonza).

Techniques: Comparison

Journal: ACS Biomaterials Science & Engineering

Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers

doi: 10.1021/acsbiomaterials.5c02175

Figure Lengend Snippet: 7ACC2 inhibition of MPC reduces oxygen consumption and TCA cycle activity. Bioenergetic profile of PC3 (a, b) and FaDu (c, d) cells treated with 7ACC2. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were monitored at 37 °C for 2 h, reported as percentage change over baseline following treatment. Data are presented as mean ± SD ( n = 3 per treatment group). Relative levels of succinate were observed in PC3 (e) and FaDu (f) cells after 7ACC2 (10 μM) treatment for 24 h. Data derived from three independent replicates, presented as mean ± SD. Statistical differences determined by one-way ANOVA with a Tukey multiple comparison test.

Article Snippet: FaDu (HPV(−) HNSCC) and PC3 (prostate cancer) cell lines were sourced from the American Type Culture Collection (ATCC) and routinely tested for mycoplasma contamination (Lonza).

Techniques: Inhibition, Activity Assay, Derivative Assay, Comparison